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In Situ Localization of N and C Termini of Subunits of the Flagellar Nexin-Dynein Regulatory Complex (N-DRC) Using SNAP Tag and Cryo-electron Tomography

Cryo-electron tomography (cryo-ET) has reached nanoscale resolution for in situ three-dimensional imaging of macromolecular complexes and organelles. Yet its current resolution is not sufficient to precisely localize or identify most proteins in situ; for example, the location and arrangement of com...

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Detalhes bibliográficos
Publicado no:J Biol Chem
Main Authors: Song, Kangkang, Awata, Junya, Tritschler, Douglas, Bower, Raqual, Witman, George B., Porter, Mary E., Nicastro, Daniela
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Biochemistry and Molecular Biology 2015
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4342452/
https://ncbi.nlm.nih.gov/pubmed/25564608
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/jbc.M114.626556
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