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A simple real-time assay for in vitro translation
A high-throughput assay for real-time measurement of translation rates in cell-free protein synthesis (SNAP assay) is described. The SNAP assay enables quantitative, real-time measurement of overall translation rates in vitro via the synthesis of O(6)-alkylguanine DNA O(6)-alkyltransferase (SNAP). S...
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| Publicado no: | RNA |
|---|---|
| Main Authors: | , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Cold Spring Harbor Laboratory Press
2015
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4338355/ https://ncbi.nlm.nih.gov/pubmed/25525154 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1261/rna.047159.114 |
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