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A simple real-time assay for in vitro translation

A high-throughput assay for real-time measurement of translation rates in cell-free protein synthesis (SNAP assay) is described. The SNAP assay enables quantitative, real-time measurement of overall translation rates in vitro via the synthesis of O(6)-alkylguanine DNA O(6)-alkyltransferase (SNAP). S...

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Bibliografiska uppgifter
I publikationen:RNA
Huvudupphovsmän: Capece, Mark C., Kornberg, Guy L., Petrov, Alexey, Puglisi, Joseph D.
Materialtyp: Artigo
Språk:Inglês
Publicerad: Cold Spring Harbor Laboratory Press 2015
Ämnen:
Länkar:https://ncbi.nlm.nih.gov/pmc/articles/PMC4338355/
https://ncbi.nlm.nih.gov/pubmed/25525154
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1261/rna.047159.114
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