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Plasmid ColE1 as a Molecular Vehicle for Cloning and Amplification of DNA

DNA fragments obtained from EcoRI endonuclease digestion of bacteriophage ϕ80pt190 (trp(+)) and the plasmid ColE1 were covalently joined with polynucleotide ligase. Transformation of Escherichia coli trp(-) strains to tryptophan independence with the recombined DNA selected for reconstituted ColE1 p...

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Detaylı Bibliyografya
Asıl Yazarlar: Hershfield, Vickers, Boyer, Herbert W., Yanofsky, Charles, Lovett, Michael A., Helinski, Donald R.
Materyal Türü: Artigo
Dil:Inglês
Baskı/Yayın Bilgisi: 1974
Konular:
Online Erişim:https://ncbi.nlm.nih.gov/pmc/articles/PMC433792/
https://ncbi.nlm.nih.gov/pubmed/4610576
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