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Plasmid ColE1 as a Molecular Vehicle for Cloning and Amplification of DNA
DNA fragments obtained from EcoRI endonuclease digestion of bacteriophage ϕ80pt190 (trp(+)) and the plasmid ColE1 were covalently joined with polynucleotide ligase. Transformation of Escherichia coli trp(-) strains to tryptophan independence with the recombined DNA selected for reconstituted ColE1 p...
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| Publicado no: | Proc Natl Acad Sci U S A |
|---|---|
| Main Authors: | , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
National Academy of Sciences
1974
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC433792/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/4610576/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.71.9.3455 |
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