Plasmid ColE1 as a Molecular Vehicle for Cloning and Amplification of DNA

DNA fragments obtained from EcoRI endonuclease digestion of bacteriophage ϕ80pt190 (trp(+)) and the plasmid ColE1 were covalently joined with polynucleotide ligase. Transformation of Escherichia coli trp(-) strains to tryptophan independence with the recombined DNA selected for reconstituted ColE1 p...

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Pubblicato in:	Proc Natl Acad Sci U S A
Autori principali:	Hershfield, Vickers, Boyer, Herbert W., Yanofsky, Charles, Lovett, Michael A., Helinski, Donald R.
Natura:	Artigo
Lingua:	Inglês
Pubblicazione:	National Academy of Sciences 1974
Soggetti:	Biological Sciences: Biochemistry
Accesso online:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC433792/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/4610576/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.71.9.3455
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