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RNA Polymerase Assembly In Vitro. Temperature Dependence of Reactivation of Denatured Core Enzyme

The Escherichia coli RNA polymerase core molecule, after denaturation in 6 M guanidine hydrochloride, can be completely reactivated in the absence of sigma subunit. Reactivation is temperature dependent. At 4° a renatured-inactive preparation is formed that has most of the secondary structure of the...

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Pubblicato in:Proc Natl Acad Sci U S A
Autori principali: Harding, John D., Beychok, Sherman
Natura: Artigo
Lingua:Inglês
Pubblicazione: National Academy of Sciences 1974
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Accesso online:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC433779/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/4610575/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.71.9.3395
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