RNA Polymerase Assembly In Vitro. Temperature Dependence of Reactivation of Denatured Core Enzyme
The Escherichia coli RNA polymerase core molecule, after denaturation in 6 M guanidine hydrochloride, can be completely reactivated in the absence of sigma subunit. Reactivation is temperature dependent. At 4° a renatured-inactive preparation is formed that has most of the secondary structure of the...
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| Publicado no: | Proc Natl Acad Sci U S A |
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| Principais autores: | , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
National Academy of Sciences
1974
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC433779/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/4610575/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.71.9.3395 |
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