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RNA Polymerase Assembly In Vitro. Temperature Dependence of Reactivation of Denatured Core Enzyme
The Escherichia coli RNA polymerase core molecule, after denaturation in 6 M guanidine hydrochloride, can be completely reactivated in the absence of sigma subunit. Reactivation is temperature dependent. At 4° a renatured-inactive preparation is formed that has most of the secondary structure of the...
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| Главные авторы: | , |
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| Формат: | Artigo |
| Язык: | Inglês |
| Опубликовано: |
1974
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| Предметы: | |
| Online-ссылка: | https://ncbi.nlm.nih.gov/pmc/articles/PMC433779/ https://ncbi.nlm.nih.gov/pubmed/4610575 |
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