Reconstitution of D-Lactate-Dependent Transport in Membrane Vesicles from a D-Lactate Dehydrogenase Mutant of Escherichia coli

Membrane-bound, flavin-linked D-lactate dehydrogenase in membrane vesicles of E. coli ML 308-225 is solubilized by extraction with guanidine HCl. When membrane vesicles prepared from a D-lactate dehydrogenase mutant are treated with this extract, they regain the capacity to catalyze D-lactate oxidat...

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Detalhes bibliográficos

Publicado no:	Proc Natl Acad Sci U S A
Principais autores:	Reeves, John P., Hong, Jen-Shiang, Kaback, H. Ronald
Formato:	Artigo
Idioma:	Inglês
Publicado em:	National Academy of Sciences 1973
Assuntos:	Biological Sciences: Biochemistry
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC433632/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/4579004/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.70.7.1917
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