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Reconstitution of D-Lactate-Dependent Transport in Membrane Vesicles from a D-Lactate Dehydrogenase Mutant of Escherichia coli

Membrane-bound, flavin-linked D-lactate dehydrogenase in membrane vesicles of E. coli ML 308-225 is solubilized by extraction with guanidine HCl. When membrane vesicles prepared from a D-lactate dehydrogenase mutant are treated with this extract, they regain the capacity to catalyze D-lactate oxidat...

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Detalhes bibliográficos
Main Authors: Reeves, John P., Hong, Jen-Shiang, Kaback, H. Ronald
Formato: Artigo
Idioma:Inglês
Publicado em: 1973
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC433632/
https://ncbi.nlm.nih.gov/pubmed/4579004
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