Reconstitution of D-Lactate-Dependent Transport in Membrane Vesicles from a D-Lactate Dehydrogenase Mutant of Escherichia coli
Membrane-bound, flavin-linked D-lactate dehydrogenase in membrane vesicles of E. coli ML 308-225 is solubilized by extraction with guanidine HCl. When membrane vesicles prepared from a D-lactate dehydrogenase mutant are treated with this extract, they regain the capacity to catalyze D-lactate oxidat...
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| Udgivet i: | Proc Natl Acad Sci U S A |
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| Principais autores: | , , |
| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
National Academy of Sciences
1973
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC433632/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/4579004/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.70.7.1917 |
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