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Reconstitution of D-Lactate-Dependent Transport in Membrane Vesicles from a D-Lactate Dehydrogenase Mutant of Escherichia coli

Membrane-bound, flavin-linked D-lactate dehydrogenase in membrane vesicles of E. coli ML 308-225 is solubilized by extraction with guanidine HCl. When membrane vesicles prepared from a D-lactate dehydrogenase mutant are treated with this extract, they regain the capacity to catalyze D-lactate oxidat...

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Vydáno v:Proc Natl Acad Sci U S A
Hlavní autoři: Reeves, John P., Hong, Jen-Shiang, Kaback, H. Ronald
Médium: Artigo
Jazyk:Inglês
Vydáno: National Academy of Sciences 1973
Témata:
On-line přístup:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC433632/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/4579004/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.70.7.1917
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