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PCR amplification of up to 35-kb DNA with high fidelity and high yield from lambda bacteriophage templates.
A target length limitation to PCR amplification of DNA has been identified and addressed. Concomitantly, the base-pair fidelity, the ability to use PCR products as primers, and the maximum yield of target fragment were increased. These improvements were achieved by the combination of a high level of...
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| 主要作者: | |
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| 格式: | Artigo |
| 語言: | Inglês |
| 出版: |
1994
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| 主題: | |
| 在線閱讀: | https://ncbi.nlm.nih.gov/pmc/articles/PMC43341/ https://ncbi.nlm.nih.gov/pubmed/8134376 |
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