PCR amplification of up to 35-kb DNA with high fidelity and high yield from lambda bacteriophage templates.
A target length limitation to PCR amplification of DNA has been identified and addressed. Concomitantly, the base-pair fidelity, the ability to use PCR products as primers, and the maximum yield of target fragment were increased. These improvements were achieved by the combination of a high level of...
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| Vydáno v: | Proc Natl Acad Sci U S A |
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| Hlavní autor: | |
| Médium: | Artigo |
| Jazyk: | Inglês |
| Vydáno: |
National Academy of Sciences
1994
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| Témata: | |
| On-line přístup: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC43341/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8134376/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.91.6.2216 |
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