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Molecular crowding overcomes the destabilizing effects of mutations in a bacterial ribozyme

The native structure of the Azoarcus group I ribozyme is stabilized by the cooperative formation of tertiary interactions between double helical domains. Thus, even single mutations that break this network of tertiary interactions reduce ribozyme activity in physiological Mg(2+) concentrations. Here...

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Salvato in:
Dettagli Bibliografici
Pubblicato in:Nucleic Acids Res
Autori principali: Lee, Hui-Ting, Kilburn, Duncan, Behrouzi, Reza, Briber, Robert M., Woodson, Sarah A.
Natura: Artigo
Lingua:Inglês
Pubblicazione: Oxford University Press 2015
Soggetti:
RNA
Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC4333387/
https://ncbi.nlm.nih.gov/pubmed/25541198
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gku1335
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