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Disclosing Bias in Bisulfite Assay: MethPrimers Underestimate High DNA Methylation
Discordant results obtained in bisulfite assays using MethPrimers (PCR primers designed using MethPrimer software or assuming that non-CpGs cytosines are non methylated) versus primers insensitive to cytosine methylation lead us to hypothesize a technical bias. We therefore used the two kinds of pri...
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| Publicado no: | PLoS One |
|---|---|
| Main Authors: | , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Public Library of Science
2015
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4333220/ https://ncbi.nlm.nih.gov/pubmed/25692551 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0118318 |
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