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Colony hybridization: a method for the isolation of cloned DNAs that contain a specific gene.

A method has been developed whereby a very large number of colonies of Escherichia coli carrying different hybrid plasmids can be rapidly screened to determine which hybrid plasmids contain a specified DNA sequence or genes. The colonies to be screened are formed on nitrocellulose filters, and, afte...

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Detalhes bibliográficos
Main Authors: Grunstein, M, Hogness, D S
Formato: Artigo
Idioma:Inglês
Publicado em: 1975
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC433117/
https://ncbi.nlm.nih.gov/pubmed/1105573
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