Negative Subtraction Hybridization: An efficient method to isolate large numbers of condition-specific cDNAs

BACKGROUND: The construction of cDNA libraries is a useful tool to understand gene expression in organisms under different conditions, but random sequencing of unbiased cDNA collections is laborious and can give rise to redundant EST collections. We aimed to isolate cDNAs of messages induced by swit...

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Bibliographic Details
Main Authors: Ray, Anamika, Macwana, Sunita, Ayoubi, Patricia, Hall, Leo T, Prade, Rolf, Mort, Andrew J
Format: Artigo
Language:Inglês
Published: BioMed Central 2004
Subjects:
Methodology Article
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC400731/
https://ncbi.nlm.nih.gov/pubmed/15050035
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1471-2164-5-22
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