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Negative Subtraction Hybridization: An efficient method to isolate large numbers of condition-specific cDNAs
BACKGROUND: The construction of cDNA libraries is a useful tool to understand gene expression in organisms under different conditions, but random sequencing of unbiased cDNA collections is laborious and can give rise to redundant EST collections. We aimed to isolate cDNAs of messages induced by swit...
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| Main Authors: | , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
BioMed Central
2004
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC400731/ https://ncbi.nlm.nih.gov/pubmed/15050035 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1471-2164-5-22 |
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