Negative Subtraction Hybridization: An efficient method to isolate large numbers of condition-specific cDNAs

BACKGROUND: The construction of cDNA libraries is a useful tool to understand gene expression in organisms under different conditions, but random sequencing of unbiased cDNA collections is laborious and can give rise to redundant EST collections. We aimed to isolate cDNAs of messages induced by swit...

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Библиографические подробности
Опубликовано в: :BMC Genomics
Главные авторы: Ray, Anamika, Macwana, Sunita, Ayoubi, Patricia, Hall, Leo T, Prade, Rolf, Mort, Andrew J
Формат: Artigo
Язык:Inglês
Опубликовано: BMC 2004
Предметы:
Methodology Article
Online-ссылка:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC400731/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/15050035/
https://ncbi.nlm.nih.govhttps://doi.org/10.1186/1471-2164-5-22
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