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Use of mCherry Red Fluorescent Protein for Studies of Protein Localization and Gene Expression in Clostridium difficile

Fluorescent proteins are powerful reporters in biology, but most require O(2) for chromophore maturation, making them inherently difficult to use in anaerobic bacteria. Clostridium difficile, a strict anaerobe with a genomic GC content of only 29%, is the leading cause of hospital-acquired diarrhea...

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Foilsithe in:Appl Environ Microbiol
Main Authors: Ransom, Eric M., Ellermeier, Craig D., Weiss, David S.
Formáid: Artigo
Teanga:Inglês
Foilsithe: American Society for Microbiology 2015
Ábhair:
Rochtain Ar Líne:https://ncbi.nlm.nih.gov/pmc/articles/PMC4325159/
https://ncbi.nlm.nih.gov/pubmed/25527559
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.03446-14
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