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Use of mCherry Red Fluorescent Protein for Studies of Protein Localization and Gene Expression in Clostridium difficile

Fluorescent proteins are powerful reporters in biology, but most require O(2) for chromophore maturation, making them inherently difficult to use in anaerobic bacteria. Clostridium difficile, a strict anaerobe with a genomic GC content of only 29%, is the leading cause of hospital-acquired diarrhea...

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Publicado en:Appl Environ Microbiol
Autores principales: Ransom, Eric M., Ellermeier, Craig D., Weiss, David S.
Formato: Artigo
Lenguaje:Inglês
Publicado: American Society for Microbiology 2015
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Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC4325159/
https://ncbi.nlm.nih.gov/pubmed/25527559
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.03446-14
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