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Endogenous biotin-binding proteins: an overlooked factor causing false positives in streptavidin-based protein detection
Biotinylation is widely used in DNA, RNA and protein probing assays as this molecule has generally no impact on the biological activity of its substrate. During the streptavidin-based detection of glycoproteins in Lactobacillus rhamnosus GG with biotinylated lectin probes, a strong positive band of...
Zapisane w:
Wydane w: | Microb Biotechnol |
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Główni autorzy: | , , , , , , |
Format: | Artigo |
Język: | Inglês |
Wydane: |
BlackWell Publishing Ltd
2015
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Hasła przedmiotowe: | |
Dostęp online: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4321382/ https://ncbi.nlm.nih.gov/pubmed/25211245 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1111/1751-7915.12150 |
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