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Mechanism of action of nalidixic acid: Purification of Escherichia coli nalA gene product and its relationship to DNA gyrase and a novel nicking-closing enzyme

A target protein for nalidixic and oxolinic acids in Escherichia coli, the nalA gene product (Pnal), was purified to homogeneity as judged by gel electrophoresis, using an in vitro complementation assay. It is a dimer of identical 110,000-dalton subunits. A polypeptide of this molecular weight is un...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: Sugino, Akio, Peebles, Craig L., Kreuzer, Kenneth N., Cozzarelli, Nicholas R.
Format: Artigo
Sprache:Inglês
Veröffentlicht: 1977
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC432036/
https://ncbi.nlm.nih.gov/pubmed/200930
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