Method for detection of specific RNAs in agarose gels by transfer to diazobenzyloxymethyl-paper and hybridization with DNA probes.

We describe a technique for transferring electrophoretically separated bands of RNA from an agarose gel to paper strips. The RNA is coupled covalently to diazobenzyloxymethyl groups on the paper. After transfer and appropriate treatment of the paper to destroy remaining diazo groups, specific RNA ba...

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Bibliografski detalji

Izdano u:	Proc Natl Acad Sci U S A
Glavni autori:	Alwine, J C, Kemp, D J, Stark, G R
Format:	Artigo
Jezik:	Inglês
Izdano:	National Academy of Sciences 1977
Teme:	Research Article
Online pristup:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC431715/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/414220/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.74.12.5350
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