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Transfer of proteins from gels to diazobenzyloxymethyl-paper and detection with antisera: a method for studying antibody specificity and antigen structure.

We describe a rapid and very sensitive method for detecting proteins as antigens after their separation in polyacrylamide/agarose composite gels, with or without sodium dodecyl sulfate. The polyacrylamide matrix is crosslinked with a reagent that can be cleaved with periodate or alkali to facilitate...

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Bibliographic Details
Main Authors: Renart, J, Reiser, J, Stark, G R
Format: Artigo
Language:Inglês
Published: 1979
Subjects:
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC383774/
https://ncbi.nlm.nih.gov/pubmed/91164
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