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Efficient transfer of large DNA fragments from agarose gels to diazobenzyloxymethyl-paper and rapid hybridization by using dextran sulfate.

We describe a technique for transferring electrophoretically separated bands of double-stranded DNA from agarose gels to diazobenzyloxymethyl-paper. Controlled cleavage of the DNA in situ by sequential treatment with dilute acid, which causes partial depurination, and dilute alkali, which causes cle...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: Wahl, G M, Stern, M, Stark, G R
Format: Artigo
Sprache:Inglês
Veröffentlicht: 1979
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC383897/
https://ncbi.nlm.nih.gov/pubmed/291033
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