Efficient transfer of large DNA fragments from agarose gels to diazobenzyloxymethyl-paper and rapid hybridization by using dextran sulfate.
We describe a technique for transferring electrophoretically separated bands of double-stranded DNA from agarose gels to diazobenzyloxymethyl-paper. Controlled cleavage of the DNA in situ by sequential treatment with dilute acid, which causes partial depurination, and dilute alkali, which causes cle...
সংরক্ষণ করুন:
| প্রকাশিত: | Proc Natl Acad Sci U S A |
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| প্রধান লেখক: | , , |
| বিন্যাস: | Artigo |
| ভাষা: | Inglês |
| প্রকাশিত: |
National Academy of Sciences
1979
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| বিষয়গুলি: | |
| অনলাইন ব্যবহার করুন: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC383897/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/291033/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.76.8.3683 |
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