Efficient transfer of large DNA fragments from agarose gels to diazobenzyloxymethyl-paper and rapid hybridization by using dextran sulfate.

We describe a technique for transferring electrophoretically separated bands of double-stranded DNA from agarose gels to diazobenzyloxymethyl-paper. Controlled cleavage of the DNA in situ by sequential treatment with dilute acid, which causes partial depurination, and dilute alkali, which causes cle...

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Pubblicato in:	Proc Natl Acad Sci U S A
Autori principali:	Wahl, G M, Stern, M, Stark, G R
Natura:	Artigo
Lingua:	Inglês
Pubblicazione:	National Academy of Sciences 1979
Soggetti:	Research Article
Accesso online:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC383897/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/291033/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.76.8.3683
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