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Method for detection of specific RNAs in agarose gels by transfer to diazobenzyloxymethyl-paper and hybridization with DNA probes.

We describe a technique for transferring electrophoretically separated bands of RNA from an agarose gel to paper strips. The RNA is coupled covalently to diazobenzyloxymethyl groups on the paper. After transfer and appropriate treatment of the paper to destroy remaining diazo groups, specific RNA ba...

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Udgivet i:Proc Natl Acad Sci U S A
Principais autores: Alwine, J C, Kemp, D J, Stark, G R
Format: Artigo
Sprog:Inglês
Udgivet: National Academy of Sciences 1977
Fag:
Research Article
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC431715/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/414220/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.74.12.5350
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