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DNA Replication Catalyzed by Herpes Simplex Virus Type 1 Proteins Reveals Trombone Loops at the Fork

Using purified replication factors encoded by herpes simplex virus type 1 and a 70-base minicircle template, we obtained robust DNA synthesis with leading strand products of >20,000 nucleotides and lagging strand fragments from 600 to 9,000 nucleotides as seen by alkaline gel electrophoresis. ICP...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Veröffentlicht in:J Biol Chem
Hauptverfasser: Bermek, Oya, Willcox, Smaranda, Griffith, Jack D.
Format: Artigo
Sprache:Inglês
Veröffentlicht: American Society for Biochemistry and Molecular Biology 2015
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4317027/
https://ncbi.nlm.nih.gov/pubmed/25471368
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/jbc.M114.623009
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