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Antigen-specific activation and cytokine-facilitated expansion of naive, human CD8+ T cells

Antigen-specific priming of human, naïve T-cells has been difficult to assess. Due to the low initial frequency in the naïve cell pool of specific T-cell precursors, such an analysis has been obscured by the requirements for repeated stimulations and prolonged culture time. In this protocol, we desc...

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Dettagli Bibliografici
Pubblicato in:Nat Protoc
Autori principali: Wölfl, Matthias, Greenberg, Philip D
Natura: Artigo
Lingua:Inglês
Pubblicazione: 2014
Soggetti:
Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC4312138/
https://ncbi.nlm.nih.gov/pubmed/24675735
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2014.064
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