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Evidence for an intermediate with a single-strand break in the reaction catalyzed by the DNA untwisting enzyme.
The DNA untwisting enzyme relaxes covalently closed circylar DNAs by the sequential breaking (nicking) and closure of one strand of the duplex. The use of highly purified enzyme from rat liver nuclei at very high protein concentrations has permitted the detection of the nicked intermediate in the re...
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
1976
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| Acceso en liña: | https://ncbi.nlm.nih.gov/pmc/articles/PMC431141/ https://ncbi.nlm.nih.gov/pubmed/1068461 |
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