Strand breakage by the DNA untwisting enzyme results in covalent attachment of the enzyme to DNA.
Strands of DNA that have been broken by the DNA untwisting enzyme exhibit a reduced buoyant density in alkaline CsCl due to bound protein. A covalent linkage between the DNA and the enzyme was indicated by the stability of the complex in alkali (pH greaterthan 12.7), in 7 M guanidine-HCl, and at 90...
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| Publié dans: | Proc Natl Acad Sci U S A |
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| Auteur principal: | |
| Format: | Artigo |
| Langue: | Inglês |
| Publié: |
National Academy of Sciences
1977
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| Sujets: | |
| Accès en ligne: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC431737/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/198805/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.74.9.3800 |
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