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BioTAP-XL - crosslinking/tandem affinity purification to study DNA targets, RNA and protein components of chromatin associated complexes

In order to understand how chromatin complexes function in the nucleus, it is important to obtain a comprehensive picture of their protein, DNA, and RNA components and their mutual interactions. Here we present a chromatin cross-linking approach (BioTAP-XL) which utilizes mass-spectrometry to identi...

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Bibliographic Details
Published in:Curr Protoc Mol Biol
Main Authors: Alekseyenko, Artyom A., McElroy, Kyle A., Kang, Hyuckjoon, Zee, Barry M., Kharchenko, Peter V., Kuroda, Mitzi I.
Format: Artigo
Language:Inglês
Published: 2015
Subjects:
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC4308290/
https://ncbi.nlm.nih.gov/pubmed/25559106
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/0471142727.mb2130s109
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