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BioTAP-XL - crosslinking/tandem affinity purification to study DNA targets, RNA and protein components of chromatin associated complexes

In order to understand how chromatin complexes function in the nucleus, it is important to obtain a comprehensive picture of their protein, DNA, and RNA components and their mutual interactions. Here we present a chromatin cross-linking approach (BioTAP-XL) which utilizes mass-spectrometry to identi...

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Detalhes bibliográficos
Publicado no:Curr Protoc Mol Biol
Main Authors: Alekseyenko, Artyom A., McElroy, Kyle A., Kang, Hyuckjoon, Zee, Barry M., Kharchenko, Peter V., Kuroda, Mitzi I.
Formato: Artigo
Idioma:Inglês
Publicado em: 2015
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4308290/
https://ncbi.nlm.nih.gov/pubmed/25559106
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/0471142727.mb2130s109
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