New method for determining total calcium content in tissue applied to skeletal muscle with and without calsequestrin

We describe a new method for determining the concentration of total Ca in whole skeletal muscle samples ([Ca(T)](WM) in units of mmoles/kg wet weight) using the Ca-dependent UV absorbance spectra of the Ca chelator BAPTA (1,2-bis(2-aminophenoxy)ethane-N,N,N′,N′-tetraacetic acid). Muscle tissue was h...

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Bibliographic Details
Published in:J Gen Physiol
Main Authors: Lamboley, Cédric R.H., Kake Guena, Sandrine A., Touré, Fatou, Hébert, Camille, Yaddaden, Louiza, Nadeau, Stephanie, Bouchard, Patrice, Wei-LaPierre, Lan, Lainé, Jean, Rousseau, Eric C., Frenette, Jérôme, Protasi, Feliciano, Dirksen, Robert T., Pape, Paul C.
Format: Artigo
Language:Inglês
Published: The Rockefeller University Press 2015
Subjects:
Methods and Approaches
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC4306712/
https://ncbi.nlm.nih.gov/pubmed/25624449
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1085/jgp.201411250
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