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New method for determining total calcium content in tissue applied to skeletal muscle with and without calsequestrin
We describe a new method for determining the concentration of total Ca in whole skeletal muscle samples ([Ca(T)](WM) in units of mmoles/kg wet weight) using the Ca-dependent UV absorbance spectra of the Ca chelator BAPTA (1,2-bis(2-aminophenoxy)ethane-N,N,N′,N′-tetraacetic acid). Muscle tissue was h...
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Publicado no: | J Gen Physiol |
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Main Authors: | , , , , , , , , , , , , , |
Formato: | Artigo |
Idioma: | Inglês |
Publicado em: |
The Rockefeller University Press
2015
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Assuntos: | |
Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4306712/ https://ncbi.nlm.nih.gov/pubmed/25624449 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1085/jgp.201411250 |
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