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Overexpression, purification, crystallization and preliminary X-ray crystallographic analysis of SF173 from Shigella flexneri

Shigella flexneri is a Gram-negative, anaerobic bacterium in the genus Shigella that can cause diarrhoea in humans. SF173, a hypothetical protein from S. flexneri 5a strain M90T, has been cloned, overexpressed, purified and crystallized as a part of laboratory-scale structural genomics project. The...

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Detalhes bibliográficos
Publicado no:Acta Crystallogr F Struct Biol Commun
Main Authors: Kim, Ha-Neul, An, Jeong-Gi, Lee, Yoo-Sup, Seok, Seung-Hyeon, Yoo, Hee-Seop, Seo, Min-Duk
Formato: Artigo
Idioma:Inglês
Publicado em: International Union of Crystallography 2015
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4304748/
https://ncbi.nlm.nih.gov/pubmed/25615969
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S2053230X14025941
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