Protein expression, crystallization and preliminary X-ray crystallographic analysis of the isolated Shigella flexneri VapC toxin

Upon release from the stable complex formed with its antitoxin VapB, the toxin VapC (MvpT) of the Gram-negative pathogen Shigella flexneri is capable of globally down-regulating translation by specifically cleaving initiator tRNA(fMet) in the anticodon region. Recombinant Shigella flexneri VapC(D7A)...

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Bibliografske podrobnosti
Main Authors: Xu, Kehan, Dedic, Emil, Cob-Cantal, Patricia, Dienemann, Christian, Bøggild, Andreas, Winther, Kristoffer S., Gerdes, Kenn, Brodersen, Ditlev E.
Format: Artigo
Jezik:Inglês
Izdano: International Union of Crystallography 2013
Teme:
Crystallization Communications
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC3702320/
https://ncbi.nlm.nih.gov/pubmed/23832203
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309113014012
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