Expression, purification, crystallization and preliminary crystallographic analysis of MxiH, a subunit of the Shigella flexneri type III secretion system needle

A monodisperse truncation mutant of MxiH, the subunit of the needle from the Shigella flexneri type III secretion system (TTSS), has been overexpressed and purified. Crystals were grown of native and selenomethionine-labelled MxiH(CΔ5) and diffraction data were collected to 1.9 Å resolution. The cry...

Deskribapen osoa

Gorde:
Xehetasun bibliografikoak
Egile Nagusiak: Deane, Janet E., Cordes, Frank S., Roversi, Pietro, Johnson, Steven, Kenjale, Roma, Picking, William D., Picking, Wendy L., Lea, Susan M., Blocker, Ariel
Formatua: Artigo
Hizkuntza:Inglês
Argitaratua: International Union of Crystallography 2006
Gaiak:
Crystallization Communications
Sarrera elektronikoa:https://ncbi.nlm.nih.gov/pmc/articles/PMC1894743/
https://ncbi.nlm.nih.gov/pubmed/16511329
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309106006555
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