Expression, purification, crystallization and preliminary crystallographic analysis of MxiH, a subunit of the Shigella flexneri type III secretion system needle

A monodisperse truncation mutant of MxiH, the subunit of the needle from the Shigella flexneri type III secretion system (TTSS), has been overexpressed and purified. Crystals were grown of native and selenomethionine-labelled MxiH(CΔ5) and diffraction data were collected to 1.9 Å resolution. The cry...

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Main Authors: Deane, Janet E., Cordes, Frank S., Roversi, Pietro, Johnson, Steven, Kenjale, Roma, Picking, William D., Picking, Wendy L., Lea, Susan M., Blocker, Ariel
Format: Artigo
Jezik:Inglês
Izdano: International Union of Crystallography 2006
Teme:
Crystallization Communications
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC1894743/
https://ncbi.nlm.nih.gov/pubmed/16511329
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309106006555
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