Expression, purification, crystallization and preliminary crystallographic analysis of MxiH, a subunit of the Shigella flexneri type III secretion system needle

A monodisperse truncation mutant of MxiH, the subunit of the needle from the Shigella flexneri type III secretion system (TTSS), has been overexpressed and purified. Crystals were grown of native and selenomethionine-labelled MxiH(CΔ5) and diffraction data were collected to 1.9 Å resolution. The cry...

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Bibliografiske detaljer
Main Authors: Deane, Janet E., Cordes, Frank S., Roversi, Pietro, Johnson, Steven, Kenjale, Roma, Picking, William D., Picking, Wendy L., Lea, Susan M., Blocker, Ariel
Format: Artigo
Sprog:Inglês
Udgivet: International Union of Crystallography 2006
Fag:
Crystallization Communications
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC1894743/
https://ncbi.nlm.nih.gov/pubmed/16511329
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309106006555
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