Direct Mutagenesis of Thousands of Genomic Targets Using Microarray-Derived Oligonucleotides

[Image: see text] Multiplex Automated Genome Engineering (MAGE) allows simultaneous mutagenesis of multiple target sites in bacterial genomes using short oligonucleotides. However, large-scale mutagenesis requires hundreds to thousands of unique oligos, which are costly to synthesize and impossible...

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Vydáno v:ACS Synth Biol
Hlavní autoři: Bonde, Mads T., Kosuri, Sriram, Genee, Hans J., Sarup-Lytzen, Kira, Church, George M., Sommer, Morten O.A., Wang, Harris H.
Médium: Artigo
Jazyk:Inglês
Vydáno: American Chemical Society 2014
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC4304438/
https://ncbi.nlm.nih.gov/pubmed/24856730
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/sb5001565
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