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Direct Mutagenesis of Thousands of Genomic Targets Using Microarray-Derived Oligonucleotides
[Image: see text] Multiplex Automated Genome Engineering (MAGE) allows simultaneous mutagenesis of multiple target sites in bacterial genomes using short oligonucleotides. However, large-scale mutagenesis requires hundreds to thousands of unique oligos, which are costly to synthesize and impossible...
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| Publicado en: | ACS Synth Biol |
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| Main Authors: | , , , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
American Chemical
Society
2014
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| Acceso en liña: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4304438/ https://ncbi.nlm.nih.gov/pubmed/24856730 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/sb5001565 |
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