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A Highly Efficient Recombineering-Based Method for Generating Conditional Knockout Mutations
Phage-based Escherichia coli homologous recombination systems have recently been developed that now make it possible to subclone or modify DNA cloned into plasmids, BACs, or PACs without the need for restriction enzymes or DNA ligases. This new form of chromosome engineering, termed recombineering,...
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| Autori principali: | , , |
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| Natura: | Artigo |
| Lingua: | Inglês |
| Pubblicazione: |
Cold Spring Harbor Laboratory Press
2003
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| Soggetti: | |
| Accesso online: | https://ncbi.nlm.nih.gov/pmc/articles/PMC430283/ https://ncbi.nlm.nih.gov/pubmed/12618378 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1101/gr.749203 |
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