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A Highly Efficient Recombineering-Based Method for Generating Conditional Knockout Mutations
Phage-based Escherichia coli homologous recombination systems have recently been developed that now make it possible to subclone or modify DNA cloned into plasmids, BACs, or PACs without the need for restriction enzymes or DNA ligases. This new form of chromosome engineering, termed recombineering,...
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| Главные авторы: | , , |
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| Формат: | Artigo |
| Язык: | Inglês |
| Опубликовано: |
Cold Spring Harbor Laboratory Press
2003
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| Предметы: | |
| Online-ссылка: | https://ncbi.nlm.nih.gov/pmc/articles/PMC430283/ https://ncbi.nlm.nih.gov/pubmed/12618378 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1101/gr.749203 |
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