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A Highly Efficient Recombineering-Based Method for Generating Conditional Knockout Mutations

Phage-based Escherichia coli homologous recombination systems have recently been developed that now make it possible to subclone or modify DNA cloned into plasmids, BACs, or PACs without the need for restriction enzymes or DNA ligases. This new form of chromosome engineering, termed recombineering,...

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Bibliografiska uppgifter
Huvudupphovsmän: Liu, Pentao, Jenkins, Nancy A., Copeland, Neal G.
Materialtyp: Artigo
Språk:Inglês
Publicerad: Cold Spring Harbor Laboratory Press 2003
Ämnen:
Länkar:https://ncbi.nlm.nih.gov/pmc/articles/PMC430283/
https://ncbi.nlm.nih.gov/pubmed/12618378
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1101/gr.749203
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