Determining the quality and complexity of next-generation sequencing data without a reference genome

We describe an open-source kPAL package that facilitates an alignment-free assessment of the quality and comparability of sequencing datasets by analyzing k-mer frequencies. We show that kPAL can detect technical artefacts such as high duplication rates, library chimeras, contamination and differenc...

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Vydáno v:Genome Biol
Hlavní autoři: Anvar, Seyed Yahya, Khachatryan, Lusine, Vermaat, Martijn, van Galen, Michiel, Pulyakhina, Irina, Ariyurek, Yavuz, Kraaijeveld, Ken, den Dunnen, Johan T, de Knijff, Peter, ’t Hoen, Peter AC, Laros, Jeroen FJ
Médium: Artigo
Jazyk:Inglês
Vydáno: BioMed Central 2014
Témata:
Method
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC4298064/
https://ncbi.nlm.nih.gov/pubmed/25514851
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s13059-014-0555-3
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