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Size Unbiased Representative Enzymatically Generated RNAi (SURER) Library and Application for RNAi Therapeutic Screens

RNA interference (RNAi) libraries screens have become widely used for small RNA (sRNA) therapeutic targets development. However, conventional enzymatically libraries, typically prepared using the type 2 restriction enzyme MmeI, produce sRNAs between 18 and 20 bp, much shorter than the usual lengths...

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Detalhes bibliográficos
Publicado no:Nucleic Acid Ther
Main Authors: Li, Tiejun, Zhu, York Yuanyuan, Chen, Li, Sun, Yuncheng, Yuan, Jian, Graham, Michael, French, Peter
Formato: Artigo
Idioma:Inglês
Publicado em: Mary Ann Liebert, Inc. 2015
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4296746/
https://ncbi.nlm.nih.gov/pubmed/25493330
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1089/nat.2014.0514
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