Size Unbiased Representative Enzymatically Generated RNAi (SURER) Library and Application for RNAi Therapeutic Screens

RNA interference (RNAi) libraries screens have become widely used for small RNA (sRNA) therapeutic targets development. However, conventional enzymatically libraries, typically prepared using the type 2 restriction enzyme MmeI, produce sRNAs between 18 and 20 bp, much shorter than the usual lengths...

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發表在:Nucleic Acid Ther
Main Authors: Li, Tiejun, Zhu, York Yuanyuan, Chen, Li, Sun, Yuncheng, Yuan, Jian, Graham, Michael, French, Peter
格式: Artigo
語言:Inglês
出版: Mary Ann Liebert, Inc. 2015
主題:
Methods
在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC4296746/
https://ncbi.nlm.nih.gov/pubmed/25493330
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1089/nat.2014.0514
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