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Use of an Endogenous Plasmid Locus for Stable in trans Complementation in Borrelia burgdorferi
Targeted mutagenesis and complementation are important tools for studying genes of unknown function in the Lyme disease spirochete Borrelia burgdorferi. A standard method of complementation is reintroduction of a wild-type copy of the targeted gene on a shuttle vector. However, shuttle vectors are p...
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| Vydáno v: | Appl Environ Microbiol |
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| Hlavní autoři: | , , , |
| Médium: | Artigo |
| Jazyk: | Inglês |
| Vydáno: |
American Society for Microbiology
2015
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| Témata: | |
| On-line přístup: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4292500/ https://ncbi.nlm.nih.gov/pubmed/25452278 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.03657-14 |
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