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Use of an Endogenous Plasmid Locus for Stable in trans Complementation in Borrelia burgdorferi

Targeted mutagenesis and complementation are important tools for studying genes of unknown function in the Lyme disease spirochete Borrelia burgdorferi. A standard method of complementation is reintroduction of a wild-type copy of the targeted gene on a shuttle vector. However, shuttle vectors are p...

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Detaylı Bibliyografya
Yayımlandı:Appl Environ Microbiol
Asıl Yazarlar: Kasumba, Irene N., Bestor, Aaron, Tilly, Kit, Rosa, Patricia A.
Materyal Türü: Artigo
Dil:Inglês
Baskı/Yayın Bilgisi: American Society for Microbiology 2015
Konular:
Online Erişim:https://ncbi.nlm.nih.gov/pmc/articles/PMC4292500/
https://ncbi.nlm.nih.gov/pubmed/25452278
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.03657-14
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