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A Continuous Enzyme-Coupled Assay for Triphosphohydrolase Activity of HIV-1 Restriction Factor SAMHD1

The development of deoxynucleoside triphosphate (dNTP)-based drugs requires a quantitative understanding of any inhibition, activation, or hydrolysis by off-target cellular enzymes. SAMHD1 is a regulatory dNTP-triphosphohydrolase that inhibits HIV-1 replication in human myeloid cells. We describe he...

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Pubblicato in:Antimicrob Agents Chemother
Autori principali: Arnold, Laurence H., Kunzelmann, Simone, Webb, Martin R., Taylor, Ian A.
Natura: Artigo
Lingua:Inglês
Pubblicazione: American Society for Microbiology 2014
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC4291348/
https://ncbi.nlm.nih.gov/pubmed/25331707
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AAC.03903-14
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