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Engineering deamidation-susceptible asparagines leads to improved stability to thermal cycling in a lipase

At high temperatures, protein stability is influenced by chemical alterations; most important among them is deamidation of asparagines. Deamidation kinetics of asparagines depends on the local sequence, solvent, pH, temperature, and the tertiary structure. Suitable replacement of deamidated asparagi...

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Shranjeno v:
Bibliografske podrobnosti
izdano v:Protein Sci
Main Authors: Bhanuramanand, K, Ahmad, Shoeb, Rao, N M
Format: Artigo
Jezik:Inglês
Izdano: BlackWell Publishing Ltd 2014
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC4287003/
https://ncbi.nlm.nih.gov/pubmed/25043738
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/pro.2516
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