Rise-Time of FRET-Acceptor Fluorescence Tracks Protein Folding

Uniform labeling of proteins with fluorescent donor and acceptor dyes with an equimolar ratio is paramount for accurate determination of Förster resonance energy transfer (FRET) efficiencies. In practice, however, the labeled protein population contains donor-labeled molecules that have no correspon...

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Detalles Bibliográficos
Publicado en:Int J Mol Sci
Main Authors: Lindhoud, Simon, Westphal, Adrie H., van Mierlo, Carlo P. M., Visser, Antonie J. W. G., Borst, Jan Willem
Formato: Artigo
Idioma:Inglês
Publicado: MDPI 2014
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Article
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC4284793/
https://ncbi.nlm.nih.gov/pubmed/25535076
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3390/ijms151223836
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