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Rise-Time of FRET-Acceptor Fluorescence Tracks Protein Folding

Uniform labeling of proteins with fluorescent donor and acceptor dyes with an equimolar ratio is paramount for accurate determination of Förster resonance energy transfer (FRET) efficiencies. In practice, however, the labeled protein population contains donor-labeled molecules that have no correspon...

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Detalhes bibliográficos
Publicado no:Int J Mol Sci
Main Authors: Lindhoud, Simon, Westphal, Adrie H., van Mierlo, Carlo P. M., Visser, Antonie J. W. G., Borst, Jan Willem
Formato: Artigo
Idioma:Inglês
Publicado em: MDPI 2014
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4284793/
https://ncbi.nlm.nih.gov/pubmed/25535076
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3390/ijms151223836
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