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Selective incorporation of 5-hydroxytryptophan into proteins in mammalian cells
An orthogonal tryptophanyl–transfer RNA (tRNA) synthetase (TrpRS)-mutant opal suppressor tRNA(Trp) ([Formula: see text]) pair was generated for use in mammalian cells. The anticodon loop of the Bacillus subtilis tRNA(Trp) was mutated to UCA, three positions in the D arm were mutated to generate an i...
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| Main Authors: | , , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
National Academy of Sciences
2004
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| Assuntos: | |
| Acceso en liña: | https://ncbi.nlm.nih.gov/pmc/articles/PMC428441/ https://ncbi.nlm.nih.gov/pubmed/15187228 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.0307029101 |
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